Coding

Part:BBa_K4271000:Design

Designed by: Ethan Ho   Group: iGEM22_Wego_Taipei   (2022-08-27)


OPH (organophosphate hydrolase) + his-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 193
    Illegal AgeI site found at 13
    Illegal AgeI site found at 298
    Illegal AgeI site found at 433
    Illegal AgeI site found at 496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA sequence of the OPH is optimized for the protein expression on E. coli.


Source

OPH from "phosphotriesterase [Agrobacterium tumefaciens]"

References